Microbial Assay for Risk Assessment

Data provider

Budapest University of Technology and Economics, Department of Applied Biotechnology and Food Science, Environmental Microbiology and Biotechnology Group

Organisation/Data provider's nameBudapest University of Technology and Economics, Department of Applied Biotechnology and Food Science, Environmental Microbiology and Biotechnology Group
Name of contact
Dr. Molnár Mónika, Dr. Feigl Viktória
Contact details
Telephone/fax
+36-1-4632347
E-mail
mmolnar@mail.bme.hu
vfeigl@mail.bme.hu
Website
http://envirotox.hu
Compulsory sheet
Method Hungarian nameMicrobial Assay for Risk Assessment
Method name
Microbial Assay for Risk Assessment
Stage of development
after application
Information on the method
Method type
laboratory test: chronic toxicity test
Measured endpoint
Toxicity tests
growth (weight, rootlength, chlorophylle content)
Other endpoint
MTC - Microbial Toxic Conc
Number of used species
multi species test
Number of other species
11
Test organisms
Bacterium
Implementation conditions
Instrument/equipment needed to perform the measurement
Incubator, scanner or plate reader
Other necessary equipment/tools
Standard laboratory facilities
Standard materials for the test
Plates, reagents and consumables
Reagents/materials for the test
Supplied with test
Data processing level
data collection, recording, evaluation by software
Necessary qualification
specialisation not required
Type of laboratory for testing
general analytical chemistry laboratory
Implementation costs
Purchase price of the equipment
400 - 800 Euro
Total cost/measurement
80 - 200 Euro
Innovation, main features
Brief concise description explaining the innovation

A multispecies microbial assay with genetically diverse test organisms inculuding prokaryotes and a eukaryote.
User-friendly simple protocol conducted in 96 well microtitre plate and data analysis performed with a dedicated software. Test generates unique toxicity fingerprint.

Typical (recommended) application of the method

Suitable for testing chemicals and both aquatic and terrestrial environmental samples. Applications to date include testing of wastwaters, receiving waters, soils, effluents, sediments, and leachates.

Is there any Protocol
yes
Detailed Protocol

Standard Operational Protocol manual supplied with test.

SWOT (evalaution based on scores)
Costs
4-low
Time requirement
3-medium
Workload requirement
5-very low
Equipment, apparata requirement
4-low
Qualified labour
5-very low
Environmental risk and workplace risks
5-very low
Environmental reality
4-good
Adequate accuracy
5-excellent
Reproducibility
5-excellent
Cost efficiency
5-excellent
Feasibility
5-excellent
Availability
5-excellent
Well known
4-good
SWOT (evaluation in words)
Strengths

Multi-species chronic evaluation. Low cost compared to conventional ecotoxicity tests.
Assay has undergone rigorous intra-laboratory and International inter-laboratory validation.
MARA has been employed in a number of projects and is currently in use worldwide.

Weaknesses

Not a direct contact test. Further development is in progress.

Other information, references
Webpage references and DEMO reports

www.ncimb.com

Publications

Available from NCIMB, includes publications in prestigious journals and book.

Completed applications
Location of the application, country
Scotland, UK
Location of the application, town and/or region
Aberdeen
Application area
Measurement of the effect/physical-chemical properties of the chemical substance
Contaminant characterisationin in environmental element/phase/sample
General characterisation of surface water
General characterisation of soil
Environmental problem the method was applied to (old)
Early warning methods/systems
Polluted sites assessment: Screening
Polluted sites assessment: Detailed assessment method
Derivation of effect based environmental quality criteria
Environmental element the method was applied to
Environmental monitoring
Environmental scenario the method was applied to
Water habitat: polluted water
Water habitat: sediment exposed to threat by polluted surface water
Water habitat: water exposed to threat by polluted sediment
Water habitat: soil exposed to threat by polluted sediment (flood, erosion)
Environmental element/phase the method was applied to
saturated soil extract
unsaturated soil extract
pore water
subsurface water
surface water
surface water sediment
leachate
Properties of the datasheet
Datasheet id (original)
718
Creator
Dr Kirit Wadhia
Status
Verified
Adatlap típusaBiological, ecotoxicological assessment and monitoring methods
Létrehozás
Módosítás